摘要
建立一种测定人血清肌红蛋白 ( Mb)的快速双位点酶免疫分析 ( ELISA) ,仅需 30 min即可给出结果 ,用以早期快速诊断急性心肌梗死 ( AMI)。要点为取得高亲和力、高滴度和高特异性的抗人 Mb抗体 ,Mb-ELISA的各项质量控制指标均符合要求。经初步临床使用 ,9例确诊为 AMI的患者 ,血清 Mb浓度为 2 4 4-955ng/m L;而7例非 AMI的患者 ,Mb均在 32 -78ng/m L正常范围之内 ,正常上限值定为 1 0 0 ng/m
A two-site sandwich ELISA of serum myoglobin(Mb) measurement has been established for rapid diagnosis of AMI, which can give the end results only in 30 minutes. The main key point to develop such a rapid test is due to having raised anti-Mb antibody with high avidity, titer and specificity. All the parameters of Mb-ELISA have fulfilled the demands of quality control. The Mb upper cutoff value has been determined as 100ng/mL. Preliminary clinical use has shown that serum Mb concentration ranged 244-955ng/mL in 9 patients definitely diagnosed as AMI while Mb value were normal(32-78 ng/mL)in 7 cases with non-AMI.
出处
《标记免疫分析与临床》
CAS
2001年第2期92-95,共4页
Labeled Immunoassays and Clinical Medicine
