机械拉伸对人牙周膜成纤维细胞Ⅰ型胶原及蛋白合成的影响

Effects of Mechanical Stretch on Collagen Type Ⅰ and Total Protein Synthesis of Human Periodontal Ligament Fibroblasts

目的 :研究拉伸应变对人牙周膜成纤维细胞Ⅰ型胶原及蛋白合成的影响。方法 :应用酶联免疫吸附实验及考马斯亮蓝微板法对拉伸应变下的细胞合成代谢变化进行观察 ,应变范围为 1%～ 8%。结果 :在 0 .5 %～ 8%拉伸应变范围内 ,人牙周膜成纤维细胞Ⅰ型胶原合成无明显变化 ,但 1%～ 8%拉伸应变作用下细胞蛋白合成减少 ,其中2 %拉伸应变影响最明显。结论

Objective:The aim of this study is to investigate the effects of mechanical stretch on typeⅠcollagen and total protein synthesis of human periodontal ligament fibroblasts (PDLFs). Methods:Enzyme link immunosorbent assay (ELISA) and Coomassie brilliant blue stain method were used to examine the anabolism of PDLFs growing on the flexible culture plates subjected to mechanical stretch. The five loading devices of PDLFs were designed and the stain varied from 1% to 8%. Results:Mechanical stretch had no effect on type Ⅰ collagen synthesis. However, in the range of 1% to 8% of stretch, the protein synthesis was reduced. The mechanical stretching of 2% strain affected the protein synthesis most obviously. Conclusion:Mechanical stretch can modify the composition of extracellular matrix of PDLFs.