反义端粒酶RNA抑制胶质瘤细胞系BT-325细胞端粒酶活性表达

Telomerase activity in human astrocytoma cell lines BT-325 is inh ibited by antisense telomerase RNA

目的 :观察反义端粒酶RNA对BT - 32 5细胞的作用 ,探讨端粒酶在恶性胶质瘤发生过程中的可能作用及反义端粒酶RNA在恶性胶质瘤基因治疗中的意义。方法 :经脂质体介导的方法将pBBS 2 12 -hTR(反义端粒酶RNA真核表达载体 )导入BT - 32 5细胞中 ,细胞克隆转移扩大培养后 ,采用TRAP -PAGE电泳、电镜和TUNEL技术检测BT - 32 5 /pBBS 2 12 -hTR细胞的端粒酶活性表达、细胞凋亡的情况。结果 :与对照组比较 ,反义端粒酶RNA显著抑制BT - 32 5细胞端粒酶活性、诱发细胞发生凋亡。结论 :转染反义端粒酶RNA在封闭细胞端粒酶活性表达的同时 ,可促进BT - 32 5细胞凋亡。在研究恶性胶质瘤发病机制及治疗方面做了有益的探讨。

Objective:To observe the effect of antise ns e telomerase RNA on BT-325 cells and to investigate the role of antisense telom erase RNA in astrocytoma treatment.Methods:Antihygromycin antisense telomerase RNA eu caryotic expression vector,pBBS 212/hTR,was introduced into human astrocytoma c ell lines BT 325 by using lipofectin mediation.TUNEL,TRAP PAGE and electron mi croscope were used to simultaneously determine BT 325/pBBS 212 hTR apoptosis and telomerase activity after hygromycin screening and cloning.Results:Compared with control,cell apoptosis rose si gnificantly in the course of antisense telomerase RNA inhibtion of BT 325 telom erase activity.Conclusion:Antisense telomerase RNA can promote BT 325 apoptosis by blocking telomerase activity.This is valuable to study astro cytoma genesis and to find the cure.