肝细胞肝癌中凋亡和增殖细胞核抗原的表达及意义

Relationship between apoptosis and PCNA expression in hepatocellular carcinoma

目的 :探讨细胞凋亡、增殖细胞核抗原 (PCNA)与肝细胞肝癌 (HCC)发生、发展的关系。方法 :利用DNA缺口末端标记技术 (TUNEL)和免疫组织化学方法 ,检测 33例肝细胞肝癌中自发性细胞凋亡和PCNA的表达。结果 :TUNEL染色凋亡阳性细胞为胞核呈蓝紫色颗粒 ,PCNA染色阳性为胞核呈棕黄色颗粒状。肝癌组织的凋亡指数AI(0 .40 )和PCNA指数PI(2 6 .75 )都明显高于癌旁肝组织的AI(0 .11)和PI(7.6 3) ,并且在包膜受侵犯者及低分化癌组织中AI和PI都明显增高 (P <0 .0 1)。此外 ,AI与PI呈高度正相关 (r=0 .8,P <0 .0 1)。结论 :细胞凋亡和PCNA参与HCC的发生和发展 ,随着HCC分化程度的降低 ,凋亡指数和PCNA指数显著增加。

Objective:To study the relationship betwe en apoptosis and PCNA expression and progression of hepatocellular carcinoma.Methods:33 HCCs samples were examined apoptosis and expre ssion of proliferating cell nuclear antigen(PCNA) by in situ end labeling ( T UNEL) and immunohistochemistry respectively.Results: The results showed that apoptosis index(AI) and PCNA index(PI)were significantly higher in tumor tissues than those in tumor surrounding tissues(P< 0.01 ).H CCs with infiltration and poor differentiation had significantly higher AI and PI than those without infiltration and well-differentiation (P<0.01).Moreov er,AI was positively related with PI(r=0.8,P<0.01).Conc lusion:Apoptosis and PCNA are associated with progression of hepat ocellular carcinoma.The proliferation rate and the incidence of apoptosis increa se significantly as the differentiation grade of HCC become lower.