吸水链霉菌NND-52菌株胞内抗生素M1分离、纯化及结构确定

Extraction, purification and structure elucidation of antibiotic M1 in mycelia of Streptomyces hygroscopicus NND-52

吸水链霉菌 NND- 5 2菌丝体 ,经溶媒浸提 ,粗结晶 ,梯度洗脱的硅胶柱层析分离 ,纯化 ,得到主要组分 M1,通过对其基本理化性质分析和 UV、IR、1 H- NMR、1 3C- NMR、DEPT等波谱分析 ,确定其结构与阿扎霉素 B相同 。

Antibiotic M1 was isolated from the mycelia of Streptomyces hygroscopicus NND 52 by alcohol extraction, crystallization and silica gel column chromatography. Basic physical & chemical characteristics and UV, IR, 1H NMR, 13 C NMR, DEPT spectra data showed antibiotic M1 is identical with azalomycin B ( elaiophylin ). It is a symmetrical macrolide antibiotic with 16 membered unsaturated dilactone ring.