RP-HPLC法测定不同部位灵芝中灵芝酸B含量

Determination of ganoderic acid B in different parts of Ganoderma lucidum

目的 测定灵芝中具有苦味的成分灵芝酸 B在灵芝中不同部位的含量。方法 采用 RP- HPL C法测定灵芝菌盖的表皮层、木栓层、菌柄、孢子粉、菌盖子实体等部位中灵芝酸 B的含量。结果 灵芝药材中灵芝酸 B主要集中在灵芝的表皮部位 ,其它部位则较少。结论 为寻找灵芝的有效成分分布规律提供了客观依据。

Object To explore the distribution of the bitter principle ganoderic acid B in different parts of Ganoderma lucidum (Leyss ex Fr ) Karst Methods Ganoderic acid B in epidermis, phellem, stipe, spore and pileus were determined by RP HPLC Results Ganoderic acid B was found to be mainly concentrated in the entire surface of the basidiocarp, other parts contained much less amount Conclusion Result of the study may provide references for the search of such active principle from this fungus