呼吸道感染患儿粒细胞集落刺激因子的测定意义

Determination and Significance of Granulocyte Colony Stimulating Factors in Children with Respiratory Airway Infection

目的 探讨粒细胞集落刺激因子 (G CSF)在呼吸道感染个体中的变化。方法 采用双抗夹心酶联免疫法和免疫单扩法对 189例呼吸道感染急性期及 5 0例治疗后 5～ 7d患儿和 30例健康儿童进行血G CSF和C反应蛋白 (CRP)检测 ,同步外周血白细胞计数。结果 血G CSF阳性 10 5 /189例 (5 5 .5 6 % ) ,CRP阳性 6 8/189例 (35 .98% ) ,同一病人G CSF和CRP重叠阳性 45 /189例 (2 3.81% )。治疗后 5～ 7d复查 5 0例CRP全部转阴 ,G CSF 16 /5 0例 (32 % )持续阳性。结论 G CSF水平在急性感染时增高 ,对临床有一定指导意义。

Objective To explore changes of the serum granulocyte colony-stimulating factor (G-CSF) in children with acute respiratory airway infection. Methods Venous blood was obtained from 30 normal children, 189 children with acute respiratory airway infection without treatment and 50 children (from the group of 189 children) treated with antibiotics for 5～7 days. G CSF levels were determined using the double antibody sandwich enzyme linked immunosorbent assay (PAS ELISA) and C reaction protein (CRP) levels were assayed using singleradial immunodiffusion. Results Of the 189 patients with acute respiratory airway infection, 105 were positive for G-CSF and 68 were positive for CRP (>12 ug/ml). Both G-CSF and CRP were positive in 23.8% of the patients. After a 5～7 day treatment course, the CRP of 50 turned from positive to negative and the G-CSF of 16 remained positive. Conclusions Serum G-CSF levels increase in the stage of acute infection.