狼疮性肾炎外周血单个核细胞B7-1和B7-2mRNA表达异常及其与疾病活动的关系

Abnormal Expressions of B7-1 and B7-2 mRNA in Peripheral Blood Mononuclear Cells from Lupus Nephritis Patients and Their Correlation with the Disease Activity

目的：观察活动期和静止期狼疮肾炎（ＬＮ）外周血单个核细胞（ＰＢＭＣ）Ｂ７－１（ＣＤ８０）和 Ｂ７－２（ＣＤ８６）ｍＲＮＡ表达及其与疾病活动的关系，以探讨 Ｂ７－１和 Ｂ７－２ ｍＲＮＡ表达异常在 ＬＮ自身免疫发生中的作用及临床意义。方法：以正常人为对照，分离１５例活动期和１３例静止期的ＬＮ患者ＰＢＭＣ，采用半定量反转录ＰＣＲ的方法，检测ＰＢ－ＭＣ的 Ｂ７－１和 Ｂ７－２ ｍＲＮＡ表达。Ｂ７－１和 Ｂ７－２ ｍＲＮＡ表达与 ＳＬＥ疾病活动评分指数（ＳＬＥＤＡＩ）的相关关系用直线相关分析。结果：静止期 ＬＮ组 Ｂ７－１和 Ｂ７－２ ｍＲＮＡ表达与正常人对照组相比无显著性差异（Ｐ＞０．０５），活动期ＬＮ组Ｂ７－１ｍＲＮＡ有明显的上升（Ｐ＜０．０５），Ｂ７－２ ｍＲＮＡ表达增高更加显著（Ｐ＜ｏ．０１）。Ｂ７－２ ｍＲＮＡ的增高与ＳＬＥＤＡＩ呈直线正相关关系（ｒ＝０．８６４１，Ｐ＜０.０５）。结论：活动期狼疮性肾炎存在着Ｂ７ｍＲＮＡ表达的异常，Ｂ７－２ｍＲＮＡ的增高与 ＳＬＥＤＡＩ呈直线正相关关系。

Objective: To investigate the expressions of B7-1 (CD80) and B7-2 (CD86 ) mRNA in peripheral blood mononuclear cells (PBMC) from patients with static and active lupus nephritis (LN) and their correlations with the disease activity,and to evaluate the clinical and autoimmune significance of B7-1 and B7-2 mRNA abnormal expressions in LN. Methods: PBMCs from l5 patients with static LN and 13 patients with active LN were isolated and B7-l and B7-2 mRNA expressions were determined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). The correlation between the expressions of B7- 1 and B7-2 mRNA and SLE disease activity index (SLEDAI) was analyzed with linear correlative analysis. Results= Compared with the healthy control group, there was no significant difference in B7-1 and B7-2 mRNA expressions in the static LN group(P>0. 05). But in the active LN group, the expressions of B7-1 and B7-2 mRNA were significantly higher (P<0. 05 and P<0. 01 respectively). There was a positive linear correlation between the elevation of B7-2 mRNA expression and SLEDAI (r = 0. 864l, P < 0. 05 ) Conclusion: Active LN patients have abnormal B7-2 mRNA expression and there is a positive linear correlation between the elevation of B7-2 mRNA expression and SLE disease activity.