巨噬细胞源性与肌源性泡沫细胞的不同特性研究

Studies on Differences Between Macrophage-derived and Smooth Muscle Cell-derived Foam Cell

目的：动脉粥样硬化损伤中的泡沫细胞来源于巨噬细胞和平滑肌细胞，本实验在离体细胞培养的基础上探讨了两种来源泡沫细胞的不同特性。方法与结果：选择Ｃ５７ＢＬ／６Ｊ小鼠腹膜巨噬细胞与猪主动脉平滑肌细胞，在氧化低密度脂蛋白（Ｏｘｉｄｉｚｅｄ ｌｏｗ ｄｅｎｓｉｔｙ ｌｉｐｏｐｒｏｔｅｉｎ，ＯＬＤＬ）环境中孵育一定时间后，可形成特征性的泡沫样细胞。其中，巨噬细胞源性泡沫样细胞的形成环境为 １０ ｍｇ·Ｌ ＯＬＤＬ，作用时间为 ９６ ｈ，细胞内总胆固醇含量为 ７７．６±８．３ｍｇ·ｇ，胆固醇酯在总胆固醇中的构成比为５３.１％ ，胆固醇含量受消斑肽影响不大，第８ｄ开始大量死亡；而肌源性泡沫样细胞则不同，形成环境为 １５ ｍｇ·ＬＯＬＤＬ，作用时间为 ７２ ｈ，总胆固醇含量为 １８７．３±９．２ ｍｇ·ｇ，胆固醇酯构成比为 ６４．１％，胆回醇含量受消斑肽影响较大，第 ６ ｄ开始大量死亡。结论：两种来源的泡沫细胞在产生环境、形成速度、胆固醇含量、生存时间和逆转性等方面可能存在着一定的差异。

Objective: Foam cells in atherosclerotic lesions derive from macrophages and smooth muscle cells. In this study, some pathological differences between the two kinds of foam cells were studied in vitro. Methods & Results: Foam-like cells were generated by incubating the peritoneal macrophages from C57BL./6J mouse or porcine aorta smooth muscle cells with oxidized low density lipoprotein (OLDL). Macrophages were incubated with 10 mg·L OLDL for 96 h. The total intracellular cholesterol was 77. 6 ±8.3 mg·g in which the cholesterol esters was 53.1%. The cholesterol content was not affected remarkably by Xiaoban-peptide. Most foam like cells began to die on the 8th day. The smooth muscle cells were incubated with 15 mg·Ｌ OLDL for 72 h.The total cholesterol was 187.3 ±9.2 mg·g, with 64. 1% being cholesterol esters. The cholesterol was influenced considerably by Xiaoban-peptide. large number of foam-like cells began to die on the 6th day. Conclusion: These results suggested that two kinds of foam cells were different in their developing environment and speeds, cholesterol contents, viabilities and reversibilities.