复黄生肌愈创油膏减少皮肤创面瘢痕形成的作用机理研究

Mechanism Study on "Compound Rhubarb Granulation-Promoting and Wound-Healing Ointment" in Reducing Cicatrization of Skin Wounds

为探讨以祛瘀补虚生肌法组方的外用复黄生肌愈创油膏 (简称复黄膏 ,主要由大黄、蛋黄油、血竭、珍珠粉等组成 )减少皮肤创面瘢痕形成的作用机理 ,造成背面皮肤局部创伤大鼠模型 ,分别采用放射免疫法、免疫组化法和RT -PCR法动态观测其对大鼠创面渗液中透明质酸 (HA)含量、创面组织中Ⅰ、Ⅲ型胶原含量以及创面修复组织中TGF -β1 mRNA表达水平的影响 ,并与白玉膏 (中医传统生肌法 )和凡士林 (临床常规用药 )作比较。结果显示 :复黄膏可明显提高HA含量 (并维持一个较高水平 )和Ⅲ /Ⅰ型胶原比例 ,对TGF -β1 mRNA表达有良好的调控作用 ,功效优于白玉膏和凡士林。

In order to explore the functional mechanism of 'Compound Rhubarb Granulation Promoting and Wound Healing Ointment' invented by the method to expel stasis,reinforce deficiency and promote granulation('Compound Rhubarb Ointment' in brief,mainly composed of Radix Rhei,Yolk oil,Resina Draconis,pearl powder,etc.) in reducing cicatrization of the skin wounds,the rat models were made with tropical lesion on the dorsal skin to dynamically observe its influence on hyaluronic acid(HA) content in wound effusion of the rats,the content of collagen Ⅰ and Ⅲ in wound tissues and the expressing level of TGF β 1mRNA in the repair tissues of the wounds by RIA,immunohistochemical method and RT PCR,in comparison with 'White Jade Ointment'(traditional granulation promoting method in TCM) and Vaseline (clinical routine drug).The results showed that 'Compound Rhubarb Ointment' could remarkably elevate HA content (and maintain it in a higher level),and the ratio of type Ⅰ and Ⅲ collagen,with a better regulatory function in expression of TGF β 1mRNA,better than 'White Jade Ointment' and Vaseline.The above functions can be regarded as its partial mechanism and important mechanism in reducing cicatrization of the skin wounds.