胰岛素脂质体的结构特点

STRUCTURE CHARATERISTICS OF INSULIN LIPOSOMES

目的 研究胰岛素脂质体中胰岛素所处的位置及结构特点。方法 采用激光散射测定其粒径大小与分布 ,用透射电镜测定形态 ;用HPLC法、荧光扫描、胰蛋白酶降解和聚丙烯胺凝胶电泳等实验手段研究胰岛素在脂质体中所处的位置。结果 胰岛素脂质体平均粒径为 2 18 3nm ,多分散度为 0 0 73 ,拟合度为 7 2 ,形态多为圆球或近圆球形。HPLC测定结果显示 ,胰岛素脂质体中含有胰岛素 ;荧光扫描结果显示胰岛素脂质体中无胰岛素荧光发射峰 ;胰岛素脂质体可抵抗胰蛋白酶对胰岛素的降解 ;胰岛素脂质体的聚丙烯胺凝胶电泳谱图中未见胰岛素的条带。结论 胰岛素脂质体中胰岛素被包裹在脂质体的内部 。

AIM To study the molecular location and state of insulin within insulin lipids. METHODS Particle size and distribution of insulin lipids was determined by using laser light scattering instrument, and transmission electron microscopy (TEM) was used to examine the morphology of insulin lipids. HPLC, fluorescence scanning, digestion with trypsin and polyacrylamide gel electrophoresis was used to determine the molecular localization and state of insulin in the lipids. RESULTS The mean particle size, polydispersity and sample quality were 218 3 nm, 0 073 and 7 2 respectively. The results of HPLC determination showed insulin exist in the insulin lipids. There was no insulin emission spectrum in the insulin lipids fluorescence spectrum; nearly no insulin digested in the test of insulin digestion by trypsin, and no insulin band in the insulin lipids in figure of polyacrylamide gel electrophoresis. CONCLUSION Insulin was encapsulated within lipids.