ELISA等3种方法检测血清TPIgG的比较研究

A Comparative Study on ELISA, TRUST, and TPPA for Testing Treponema Pallidum Antibody

目的 探讨ELISA方法测定血清TPIgG诊断梅毒的应用价值 ,尤其是对大批标本检测的应用。方法 随机抽取 2 4 5例卖淫嫖娼人员的血清标本 ,分别用ELISA与TRUST ,TPPA 3种方法检测。比较三者间的敏感性和特异性。结果 ELISA法与TRUST法符合率 99.18% ,ELISA对TRUST的敏感率为 99.0 3 % ,特异性99.0 3 %。ELISA与TPPA结果符合率 99.18% ,ELISA对TPPA敏感率为 99.5 1% ,特异性为 97.3 0 %。结论 3种方法比较 ,差异无显著性。

Objective To evaluate the diagnostic value of ELISA for determining TP?IgG.Methods 245 serum samples from the prostitutes were collected with random sampling method. The TP antibody was detected with ELISA, TRUST and TPPA respectively, and their sensitivity and specificity rates were compared.Results There were 99.18% in agreement, being 99.03% in sensitivity rate and 99.03% in specificity rate between ELISA and TRUST. There were 99.18% in agreement, being 99.51% in sensitivity rate and 97.30% in specificity rate between ELISA and TPPA.Conclusion There is no significant difference among three methods in detecting TP antibody but ELISA method has better practical application.