还原型谷胱甘肽和乙酰半胱氨酸对内毒素休克兔的保护作用

Protective effects of GSH or NAC on the endotoxine-shocked rabbits

目的 研究抗氧化剂还原型谷胱甘肽 (GSH)和乙酰半胱氨酸 (NAC)对兔内毒素休克早期的保护作用。方法 2 4只兔随机分为对照组、NAC组和GSH组。注射内毒素LPS30 0 μg/kg后 ,两实验组立即给予NAC和GSH ,在 0、1 5、2 5、3 5、4 5和 5 5小时分别测定收缩压、舒张压 ,并采血测乳酸 (LD)、丙二醛 (MDA)和一氧化氮 (NO)。于实验结束后取标本进行病理检查。结果 NAC组的收缩压和舒张压与对照组相比可维持于较高水平 ,GSH组在实验后期也比对照组的降幅小。两实验组的NO浓度与对照组相比基本无明显差异 ,在 5 5小时达 5 0～ 6 0 μmol/L ,比初始值增高一倍 ;而MDA和LD浓度的增高在两实验组均比对照组明显要小 ,且GSH组比NAC组效果更明显。病理检查可见三组动物的心脏、肺脏、肝脏和肾脏均有坏死和出血点。结论 内毒素休克早期给予NAC或GSH均有一定的保护作用 ,表现为减缓血压下降和过氧化损伤等。但其保护作用并不全面 。

Objective To study the protective effects of reduced glutathione(GSH) and N acetyl L cysteine(NAC) at errly stage of endotoxic shock in rabbits.Methods 24 rabbits were divided into control group,NAC group and GSH group randomly.Each group was challenged by E.Coli LPS(055∶B5) in a dose of 300μg/kg injected within 30min.The diastolic pressure and systolic pressure were recoded at the times of 0h,1 5h,2 5h,3 5h,4 5h and 5 5h respectivly.At the same time,blood samples were taken for the measurments of plasma lactic acid(LD),malondialdehyde(MDA), nitric oxide(NO).Autospy and pathological study were performaned soon after the experiment.Results The decrease of diastolic pressure and systolic pressure was fewer in NAC group than that in control group.So was in GSH group at the later stage.There was no significant difference among the three groups in NO.The concentration of MDA,LD,AST and LDH was higher in control group than that in the others.And that of GSH group is signifiantly lower than that of NAC group.Pathological study showed that there were many hemorrhagic spots and necrosis on the heart,lung,liver and kidney slices in all three groups.Conclusion Both of NAC and GSH given at early stage of endotoxic shock have some protective effects manifested by attenuating the decrease in blood pressure and suproxide injury. [