PCR检测泌尿系感染者尿大肠杆菌的探讨

Detection of urine Escherichia coli in patients with urinary infection

目的；探讨聚合酶链反应（ＰＣＲ）检测泌尿系感染患者尿内大肠杆菌的敏感性。方法：采用ＰＣＲ及细菌培养的方法检测５５例泌尿系感染患者尿内大肠杆菌，并对两种方法进行比较。结果；泌尿系感染症状反复发作而细菌培养常为阴性的患者．用ＰＣＲ法检测常为阳性；ＰＣＲ法检测阳性率明显高于细菌培养法。结论：采用ＰＣＲ法检测泌尿系感染患者尿内大肠杆菌具有较高的敏感性。

Purpose:To detect the urine Escherichia coli in patients with urinary infection. Method;PCR and bacteria culture were applied in the detection of urine Escherichia coli. Result: Positive detection rate of PCR is obviously higher than bacteria culture in the patients who have no infection by bacteria culture whereas they always have clinical symptoms of urinary infection. Conclusion:PCR is more sensitive in the detection of urine Escherichia coli in the patients with urinary infection.