不同条件培养的内皮细胞耐受流体剪切力的比较研究

Effect of Different Cultured Conditions on Endothelial Cell and Its Resistance to the Fluid Imposed Shear Stress——  Comparative Study

为提高内皮细胞 (EC)与移植物间的粘附 ,研究其可能的机制 ,我们采用改进的流室装置比较流动培养的 EC与静态培养的 EC对剪切力的耐受强度 ,预铺纤维粘连蛋白、层粘连蛋白对其粘附的影响 ,并研究了 EC骨架成分肌动蛋白丝的分布及在剪切力作用下的变化。结果显示流动培养组的细胞残留明显多于相应静态培养组 ,预铺纤维连接蛋白可显著提高静态培养 EC的残留 ,加入纤维连接蛋白和层粘连蛋白效应更明显 ,而预铺的基质对流动培养组结果影响较小。剪切力作用下 EC的肌动蛋白丝有序排列 ,并形成张力纤维。提示流动培养可显著提高 EC对剪切力的耐受 ,其机制与剪切力诱导肌动蛋白丝重建、张力纤维形成。

This study was intended to enhance the resistance of cultured endothelial cells (EC) to fluid shear stress and clarify the possible mechanism. A modified parallel plate flow apparatus was used to compare the strength between the grafts and the endothelial cells under shear stress condition and static condition. The dynamic change of cytoskeletal actin filaments and the effects of different adhesive proteins coated on the strength of EC adhesion to the glass were studied. The results showed that the number of cells retention of the shear stress-conditioned group was significantly larger than that of the static group. Precoated fibronectin and laminin significantly promoted the adherence of the EC cultured in a steady environment but seemed to have no effect on the EC cultured under flow condition. The results suggest that the vascular endothelial Cell cytoskeleton, stress fiber and the shape of EC reorganize in response to long term fluid-imposed shear stress, and the endothelial cells become tightly adhe rent to the grafts.