红细胞剪切弹性模量与表面粘度的改变对红细胞变形与取向的影响

Effects of the Alterations of Membrane Shear Elastic Modulus and Viscosity on the Deformation and Orientation of RBCs

神经氨酸酶 (唾液酸酶 )与红细胞发生生物化学作用能部分地去掉其表面电荷 ,从而引起红细胞膜微观结构发生改变 ,导致其膜剪切弹性模量和表面粘度的变化 ,分别改变其作用时间和二者的相对作用剂量 ,然后用一种在低粘切变流场中能将红细胞变形指数 DI分解为取向指数 (DI) or和小变形指数 (DI) d 的新型激光衍射法 ,分别测量经过各种处理的红细胞的小变形指数 (DI) d 和变形恢复过程即松弛过程中变形恢复到最大值 (DI) max一半的时间 t0 .5 ,并将其结果分别代入由文宗曜、严宗毅等 [1 ]提出的一种测量红细胞膜的剪切弹性模量 (E)公式和表面粘度(μm)公式。得出各种处理后的红细胞膜剪切弹性模量和表面粘度的变化规律。同时测量经过各种处理的红细胞的变形指数 DI和取向指数 (DI) or,也得到二者的变化规律。发现随着神经氨酸酶处理剂量的增加和处理时间的延长红细胞膜剪切弹性模量和表面粘度明显增大 ,而红细胞的变形指数 DI和取向指数 (DI) or却减小 ,呈现明显的反相关关系。说明红细胞膜剪切弹性模量的增大使其变形性变差 。

Neuraminidase can partly remove the surface charge of RBCs through a biochemical interaction; thus it can give rise to alterations in the microstructure of membrane, the shear elastic modulus (E) and the viscosity(μm) of membrane. Changing the time of treatment and the dose of neuraminidase and using a new ektacytometry that can separate deformation index DI into orientation index (DI)or and small deformation index (DI)d for RBCs in shear flow field of low viscosity, we measured (DI)d and the half time t0.5 when the DI recovered to half of the maximum in the process of relaxation for every treated sample. (DI)d and t0.5 were put respectively into the RBC membrane shear elastic modulus formula and the membrane viscosity formula which were put forward by Wen Zong-yao and Yan Zong-yi et al[1]. The rules of the alterations of E and μm were obtained. We also measured DI and (DI)or. It was found that E and μm increased greatly but DI and (DI)or decreased when the dose of neuraminidase and the time of treatment were increased. There was a contrary correlation between them. These data demonstrated that the increase of E and μm weakened the deformability and the ability of orientation of RBCs.