阿霉素磁性蛋白微球联合外磁场对恶性肿瘤细胞的毒性试验

The Cytotoxic Effects of the Adriamycin Magnetic Albumin Microspheres Combined with External Magnetic Fields on the Malignant Tumor Cells

为了观察恒定磁场诱导下阿霉素磁性蛋白微球体外对恶性肿瘤细胞生长的抑制作用。将体外培养的Walker- 2 5 6细胞分为阿霉素磁性微球联合磁场组 ,阿霉素磁性微球组及阿霉组三组 ,倒置显微镜观察细胞的生长状态 ,改良 MTT法检测各组细胞的抑制率。结果表明 :阿霉素磁性微球组与游离阿霉素组抑制率 (P>0 .0 5 ) ,联合磁场组抑制率明显提高 (P<0 .0 1) ,细胞形态出现显著变化。显示阿霉素磁性蛋白微球与游离阿霉素对恶性肿瘤细胞具有相似的抑制作用 。

This study sought to assess the inhibitory effects of the adriamycin magnetic albumin microspheres (ADM-MAMs) on Walker-256 malignant tumor cells in vitro induced by the permanent magnetic fields. The cultured Walker-256 cells were divided into three groups: The group of ADM-MAMs combined with magnetic fields, the group of AMD-MAMs without magnetic fields, and ADM group. The growth states of the cells were observed and photographed under the inverted microscope. The inhibitory rates(IR) were assayed by the modified MTT colorimetric method. The results showed that the IR of the ADM-MAMs group were similar to those of the ADM group (P>0. 05), but the group of ADM-MAMs combined with magnetic fields had obviously higher IR and significent changes of the cells' shapes. These findings indicate that the anticancer effect of ADM-MAMs on malignant tumor cells is similar to that of ADM, and such effect can be increased by the combined use of ADM-MAMs and external magnetic fields.