血管紧张素-Ⅱ对鼠胚心纤维母细胞癌基因和前胶原基因的影响

Effects of Ang-Ⅱ on c-fos mRNA and pro-α_1[Ⅰ]mRNA of cadiac FBC in rat embryo

目的 观察血管紧张素 - (Ang- )对大鼠胚胎心纤维母细胞 (FBC)胶原合成过程中癌基因 c- fosm RNA及前胶原基因 α1 [ ](pro- α1 [ ]) m RNA表达的影响。方法 用培养大鼠胚胎心 FBC,以荧光法检测Ang- 引起的 FBC内游离钙浓度变化 ,用 RT- PCR法检测 c- fos m RNA及 pro- α1 [ ]m RNA表达。结果 1Ang- 10 - 8～ 10 - 5使 FBC内游离钙浓度呈剂量依赖性增加 ;2 PCR显示 ,FBC经 Ang- 10 - 7～ 10 - 6 mol/ L 作用后 ,c- fos m RNA和 pro- α1 [ ]m RNA表达增强。结论 Ang- 通过活化 FBC内信号系统诱导 c- fos m RNA及pro- α1 [ ]m

Objective To observe the effects of Ang Ⅱ on expressions of c fos mRNA and pro α 1[Ⅰ]mRNA in fibroblasts (FBC) of rat embryo in the course of collagen synthesis.Methods The change of free Ca 2+ ([Ca 2+ ]i) was tested in fluorometry and the expressions of c fos mRNA and pro α 1[Ⅰ]mRNA were tested by RT PCR method in incubated FBC.Results The level of [Ca 2+ ]i in FBC and the expressions of c fos mRNA and pro α 1[Ⅰ]mRNA were enhanced with the increase of Ang Ⅱ concentration(10 -8 ~10 -5 and 10 -7 ~10 -6 mol/L).Conclusions The expressions of c-fos mRNA and pro-α_1[Ⅰ]mRNA were induced by Ang-Ⅱ activating signal system in FBC.