日本血吸虫重组BCG-Sj26GST疫苗诱导小鼠脾细胞TNF-α和IL-1变化的研究

Study on changes of TNF-α and IL-1 in spleen cells of mice induced by recombinant BCG-Sj26GST vaccine of schistosoma japonicum

目的 研究日本血吸虫重组 BCG- Sj2 6 GST疫苗对小鼠脾细胞产生 TNF-α和 IL - 1的影响。方法 实验 1分别采用 10 6 CFU和 10 8CFU疫苗皮下免疫 BAL B/C鼠 ,免疫后 8w用日本血吸虫尾蚴进行攻击感染 ,感染后 6 w剖杀小鼠 ,同时设有 PBS对照组。实验 2用 10 6 CFU疫苗皮下和静脉注射分别免疫小鼠 ,于免疫后 0、4、8、10、14和 16 w各剖杀 4只 ,分离脾脏 ,用 Sj2 6或丝裂原刺激脾细胞 ,用 EL ISA法检测脾细胞上清液中 TNF-α水平 ,用成纤维细胞增殖法检测 IL - 1的生物活性。结果 疫苗免疫 ,尾蚴攻击后 ,TNF-α和 IL - 1水平显著升高。动态观察发现皮下注射组 TNF-α和 IL - 1于免疫后 4～ 8w达最高水平 ;静脉注射组 TNF-α和 IL - 1分别于免疫后 16 w和 4～ 8w达较高水平。结论 日本血吸虫重组 BCG- Sj2 6 GST疫苗促进小鼠脾细胞较早分泌 TNF-α和IL - 1。

Objective To study the effects of recombinant BCG Sj26GST vaccine of schistosoma japonicum of TNF α and IL 1 produced by spleen cells of mice.Methods In experiment 1,BALB/C mice were immnized subcutaneously by 10 6 and 10 8 CFU vaccine respectively,challenged with Sj cercariae on 8 w of immunization and killed on 6 w of infection to separate spleens,PBS served as control;in experiment 2,after immunized subcutaneously and intravenously by 10 6 CFU vaccine respectively,4 mice were killed to separate sleens on 0,4,8,10,14 and 16 w of immunization,spleen cells were stimulated by Sj26 or mitogens,the level of TNF α in supernatant of spleen cells were detected by ELISA,that of IL 1 by fibroblast proliferative response.Results Levels of TNF α and IL 1 increased obviously by immunization with the vaccine against challenge with Sj cercariae;dynamic observation showed that TNF α and IL 1 in the subcutaneous group reached the highest level on 4~8 w;that in the intravenous group on 16 w and 4~8 w respectively.Conclusions Recombinant BCG Sj26GST vaccine of schistosoma japonicum may promote spleen cell of mice to secrete TNF α and IL 1 early,these cytokines may play an important role in protective immunity against schistosomiasis.