内切葡聚糖酶的纯化及其基本性质

Purification and Properties of Endoglucanase

纤维素酶是自然界碳素循环中的一种关键酶,它可以将纤维素性材料,转化为可直接利用的葡萄糖,并可通过其它酶和酶系统的作用转化为乙醇及蛋白质。纤维素酶是多酶体系,包含外切葡聚糖酶、内切葡聚糖酶(Cx)及β-葡萄糖苷酶。其中内切葡聚糖酶含有若干种同工酶,能将可溶性纤维素转化为还原寡糖,是纤维素酶系中的重要组分,为了深入研究Cx酶的性质,本文对其中一种Cx酶进行了分离纯化,并对其基本性质进行了研究。

Cellulase R-10 component (EC 3. 2. 1.4) derived from trichoderma viride was investigated. Endoglucanase was purified by consecutive column chromatography. The enzyme was homogeneous on being verified by HPLC and polyacrylamide gel electrophoresis. The carbohydrate of the enzyme was 4.5％ by phenol-sulfuric acid method and the molecular weight was estimated to be 41000 by SDS-polyacrylamide gel electrophoresis. The isoelectric point of the enzyme was 5.48.