DNA指纹技术在马海毛山羊育种中的应用研究

A Study of Application of DNA Fingerprint Technique in Mohair Goat Breeding

以 (CA/GATA/TCC) 5探针 ,HinfI酶切 ,研究安哥拉山羊与建昌黑山羊杂交的亲本及其F2 、F3 的DNA指纹图谱 .结果 ,在供试山羊中 ,个体平均检出⒙ 2 +0 .4条谱带 ,安哥拉山羊F1,F2 的鉴别机率分别为 1.38× 10 - 12 ,1.18× 10 - 13,0 .50× 10 - 10 ;群体内相似系数安哥拉山羊为 0 .4 539,F2 为 0 .4 0 77,F3为 0 .6111;亲子临定的父权概率W =0 .9889;安哥拉山羊与建昌黑山羊比较 ,2 .3kb和 8.

The DNA fingerprint atlas of Angora goat, Jianchang black goat,and their F 2,F 3 generations have been established by using(CA/GATA/TCC) 5 as a probe and Hinfi enzyme cutting.The results show that,the individual average 18.2+0.4 atlas bands are tested out in experimental goats,the judging probabilities of Angora goat,F 2 generation and F 3 gineration are 1.38×10 -12 ,1.18×10 -13 ,0,50×10 -10 respectively;the similar coeffcients in population of Angora goat,F 2.F 3 are 0.4539,0.4077,0.6111 respectively;the paternity probability (W) of parent offspring evalution is 0.9889;the 2.3kb and 8.6kb atlas bands are extraordnary atlas bands of Angora goat as compared with Jianchang black goat.