天然抗氧化剂Isoverbascoside诱导人胃癌MGC 803细胞分化作用研究

Differentiation of Human Gastric Cancer MGC 803 Cell Induced by Natural Antioxidant Isoverbascoside

人胃癌 MGC80 3细胞经天然抗氧化剂 Isoverbascoside(1sov)处理后 ,细胞生长和增殖呈现Isov浓度和处理时间依赖性抑制 ,群体倍增时间也相应延长 ;2 0 μmol/ L Isov处理可使细胞形态和超微结构发生正常化变化 ,集落形成率受抑 ;胃癌分化标志酶碱性磷酸酶和乳酸脱氢酶活性均呈时间依赖性下降 ,周期表现为 G1期阻滞 .表明 Isov具有诱导 MGC 80 3细胞分化的作用 。

While treated with natural antioxidant lsoverbascoside (Isov),the growth and proliferation of human gastric carcinoma MGC 803 cell were markedly inhibited and the average cell population double time was correspondingly prolonged, with dependence on concentration and treatment time of Isov. Compared with control group, the cell morphology changed toward normality and cloning efficiency on soft agar was a1so inhibited while treated with 20 μmol/L Isov. As marker enzymes of differentiation,the activities of alkaline phosphatase (AKP) and 1actate dehydrogenase (LDH) were respectively down regulated, depending on treatment time. Detected by flow cytometer, the cell cycle distribution showed G 1 arresting.These results suggested that differentiation of MGC 803 cell induced by natural antioxidant Isov and may associate to cell cycle arresting.