利用分子烙印技术分离中草药活性组分

Extracting Active Compounds from Herbs Using Molecular Imprinting Technology

用非共价法，在极性溶剂中，以丙烯酰胺作功能单体，以强极性化合物槲皮素为模板，制备了分子烙印聚合物(MIP). 液相色谱实验表明, MIP 对槲皮素具有特异的亲合性.将此MIP直接分离银杏叶提取物水解液, 得到主要含模板槲皮素及与槲皮素结构相似化合物山奈酚两种黄酮的组分.研究证实了MIP技术用于直接分离、提取中草药中具有特定药效化合物的可行性.

A molecularly imprinted polymer (MIP) was prepared using quercetin as the template and acrylamide as the hydrogen-bonding functional monomer in a polar solvent. The polymer exhibited high affinity for quercetin and good selectivity among the tested compounds. And when it was used as an extraction cartridge for the hydrolyzate of gingko leaves, it can purify the flavonoid component in the hydrolyzate, catching mainly quercetin and kaempferol. Thus it demonstrated the feasibility of using MIP in extracting specific pharmacophoric compounds directly from herbs.