摘要
The solution structure of the imidazole adduct of oxidized horse heart cytochrome c(Im cyt c) has been determined through 2D NMR spectroscopy. The 35 conformers of the family show the RMSD values to the average structure of 0.063±0.007 nm for the backbone and 0.107±0.007 nm for all heavy atoms, respectively. The secondary structure elements and the overall folding of the present structure are substantially similar to those of the solution structure of native protein. However, the replacement of the axial ligand Met 80 with the exogenous imidazole ligand induces significant conformation changes in both backbone and side chains of the residues locating in the distal axial ligand regions. The electron delocalization on the heme moiety and the magnetic susceptibility tensor are consistent with these structural features.
The solution structure of the imidazole adduct of oxidized horse heart cytochrome c(Im cyt c) has been determined through 2D NMR spectroscopy. The 35 conformers of the family show the RMSD values to the average structure of 0.063±0.007 nm for the backbone and 0.107±0.007 nm for all heavy atoms, respectively. The secondary structure elements and the overall folding of the present structure are substantially similar to those of the solution structure of native protein. However, the replacement of the axial ligand Met 80 with the exogenous imidazole ligand induces significant conformation changes in both backbone and side chains of the residues locating in the distal axial ligand regions. The electron delocalization on the heme moiety and the magnetic susceptibility tensor are consistent with these structural features.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2001年第6期947-948,共2页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 !(批准号 :2 973 10 3 0
3 9870 166)资助