摘要
It has been found that Ser His could cleave bovine serum albumin(BSA), with the most suitable pH around 6.0. The kinds of buffers have different influences on the cleavage activity. The phosphate buffer and BR buffer are the most effective. The hydroxyl group of the serine residue is the essential functional group for the cleavage, while the amide bond, imidazole, and carboxyl group of Ser His increase the cleavage activity.
It has been found that Ser His could cleave bovine serum albumin(BSA), with the most suitable pH around 6.0. The kinds of buffers have different influences on the cleavage activity. The phosphate buffer and BR buffer are the most effective. The hydroxyl group of the serine residue is the essential functional group for the cleavage, while the amide bond, imidazole, and carboxyl group of Ser His increase the cleavage activity.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2001年第8期1349-1351,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学重点项目基金 (批准号 :2 96 32 0 0 4)
访问学者基金 (批准号 :教技司 [1999] 15 3号 )
