酪氨酸、脯氨酸和组氨酸的示波极谱连续测定

Investigation on the simultaneous determination of tyrosines, proline and histidine by oscillopolarography

提出了用示波极谱法连续测定酪氨酸 ( Tyr)、脯氨酸 ( Pro)、组氨酸( His) 3种氨基酸的新方法。在 p H9.2硼酸缓冲溶液中 ,上述 3种氨基酸与镍发生配位反应 ,用示波极谱仪进行单扫描时 ,出现 3组灵敏度和分辨率均很高的二阶导数波谱 ,峰电位分别为 :Tyr - 1 .0 4 V,Pro - 1 .2 0 V,His - 1 .34V。酪氨酸、脯氨酸、组氨酸的峰电流与浓度分别在 2 .0× 1 0 -5～ 3.0× 1 0 -4 mol/L,3.2× 1 0 -6～ 2 .2× 1 0 -4 mol/L,4.0× 1 0 -5～ 2 .5× 1 0 -4 mol/L的范围内呈线性关系。该法已用于复方氨基酸注射液中酪氨酸、脯氨酸、组氨酸含量的测定。

An oscillopolarographic method for simultaneous determination of tyrosine, proline and histidine was described. In a borate buffer (pH9 2) , nickel(Ⅱ) could combine with the three amino acids and form complexes. The reduction peak potential of the complexes are -1 04v(tyr), -1.20v(pro) , -1.34v(his), respectively. The liner relationship between the concentrations and their peak heights are in the range of 3.2×10 -6 ～2.2×10 -4 mol/L(pro),4.0×10 -5 ～2.5×10 -4 mol/L(hir), and 2.0×10 -5 ～3.0×10 -4 mol/L(Tyr). The method was applied to the determination of the three amino acids in injection samples.