采用带微型预处理柱和分离柱的FIA分光光度系统同时测定硅和磷

Simultaneous determination of silica and phosphate in biological and plant materials by using HPLC colums based on flow injection spectrophotometry

用阳阴离子交换柱和流动注射分析组合 ,建立了在线预处理和预分离同时测定磷和硅的 FIA方法。用本法测定了头发、淡菜、茶叶、马尾藻和大米中的硅和磷。方法的测定范围为 1 .0 mg/ L～ 2 4 mg/ L ( PO4 3 -) ,0 .5mg/ L～ 1 2mg/ L( Si O3 2 -) ,回收率 :96.7%～ 99.0 % ( PO4 3 -) ,1 0 0 %～ 1 0 2 % ( Si O3 2 -) ;RSD( PO4 3 -) =1 .3% ,RSD( Si O3 2 -) =1 .8%。

A description of simultaneously determining silicate and phosphate in human hair, mussel,tea leaves,sargasso and rice flour is given by using a flow injection spectrophtometric system based on an on line ion exchange pretreatment to remove interfering cations and a separation column to separate silicate and phosphate ions in the sample zone.The determination ranges are 1 0 mg/L～24 mg/L for PO 4 3- and 0 5 mg/L～12 mg/L for SiO 3 2- . Recovery ranges are 96.7%～99 0% for PO 4 3- and 100%～102% for SiO 3 2- with RSD of 1.3% and 1.8%,respectively.