过氧化氢-硫唑嘌呤氧化还原反应的研究与应用

Study on redox reaction of azathioprine with hydrgen peroxide and its analytical application

研究了硫唑嘌呤水解产物与过氧化还原作用及产物的荧光特性 ,提出了高灵敏度测定硫唑嘌呤的荧光光度分析新方法。在碱性介质和沸水加热条件下 ,硫唑嘌呤的水解产物能被过氧化氢氧化 ,生成 6-嘌呤磺酸钠 ,该产物不仅具有强的荧光且稳定性好。本体系的激发波长为 2 86nm,发射波长为 397nm,荧光强度与硫唑嘌呤的含量在 0 .0 2 5～ 2 .4mg/L 范围内呈线性关系 ,检测限为0 .0 1 mg/L。该法已应用于片剂中硫唑嘌呤含量的测定。

A new spectrofluorimetric method for determination of azathioprine(6 AP) based on the redox reaction of 6 AP with H 2O 2 in the NaOH medium and boiling water bath was established. Under the optimum condition, the linear relationship between the fluorescence intensity and the concentration of 6 AP was found to be 0 025～2 4 mg/L with detection limit of 0 01 mg/L. The method was applied to determine 6 AP in tablets with satisfactory results.