反相层析分离紫杉醇的柱动力学

COLUMN DYNAMICS OF TAXOL PURIFICATION ON REVERSED PHASE CHROMATOGRAPHY

通过考察物质在液固两相之间的基本传递现象 ,建立了常压非线性柱层析过程的速率模型 .对紫杉醇在C18-硅胶柱上分离纯化过程进行了数学分析 .模型计算值与实验值吻合良好 .研究物料浓度、进料速度、层析柱大小等因素对紫杉醇层析过程的影响说明 。

Taxol, originally isolated from the bark of Taxus brevifolia, is a very important new anti-tumor drug.Reversed phase chromatography provides a rapid, efficient, and inexpensive method to separate and purify taxol. However, it is very important to scae-up the isolation process in order to obtain a large amount of taxol. From studies on the basic transport phenomena between solid phase and liquid phase, the rate model for non linear liquid chromatography under normal pressure is proposed.The process of taxol separation on C 18 - silica gel column could be described by this model very well.The numerical solution of model agrees well with experimental data.The factors affecting the separation of taxol, including feed concentration,feed rate and column size are investigated.It is confirmed that the mathematical model could be very useful for scaling-up chromatographic operation.