用新型尼龙亲和膜纯化人血浆蛋白

Purification of Proteins in Human Plasma with New Nylon Affinity Membranes

以微孔尼龙膜为基质 ,分别采用三氯三嗪和 1,4 丁二醇二甘油醚活化法制备了一系列亲和膜。首次使用平板亲和膜方法经一步操作即从人血浆中纯化得到高纯度的γ 球蛋白、纤溶酶原。电泳分析显示 ,纯化得到的人血浆γ 球蛋白 ,纯度在 83%以上 ;纯化得到的γ 球蛋白和纤溶酶原的纯度均高于市售同类产品 ;另外 ,初步探索了人血浆凝血酶的一步纯化法。该法纯化得到的凝血酶比活为 42 0NIH单位 /mg～ 42 5NIH单位

A series of affinity membranes were prepared by using microporous nylon membrane as matrix and activation method with s triazine and 1,4 butanediol diglycidyl ether, separately Three proteins with clinic value, γ globulin, plasminogen and thrombin, were purified from human plasma by one step with affinity membrane chromatography The purities of γ globulin purified were higher than 83%, and purification folds were higher than 5 The purity of γ globulin obtained was higher than that of standard human γ globulin The efficiencies of γ globulin purification with affinity membranes prepared by two activation methods were equal Plasminogen purified with affinity membranes prepared by s triazine activation was higher than that by 1,4 butanediol diglycidyl ether activation And SDS PAGE analysis showed that the purities of γ globulin and plasminogen obtained were higher than that of the commercial product In addition, the purification of thrombin from human plasma by one step with trypsin activation column and affinity membrane was studied Thrombin with specific activity of 42 NIH unit/mg and 42 5 NIH unit/mg could be obtained from human plasma through affinity membranes prepared by s triazine and 1,4 butanediol diglycidyl ether methods, respectively