羟基苯甲酸的3种异构体的毛细管区带电泳分离研究

Research on the Separation of Three Isomers of Hydroxybenzoic Acid by Capillary Zone Electrophoresis

采用十六烷基三甲基溴化铵 (CTAB)为电渗流改性剂 ,考察了邻羟基苯甲酸、间羟基苯甲酸和对羟基苯甲酸在高效毛细管区带电泳模式下的分离行为 ,研究了缓冲液的pH和添加剂甲醇的用量对这 3种同分异构体的分离、峰形和出峰顺序的影响 ,并据此优化了这 3种异构体的分离条件。

The separation behavior of o hydroxybenzoic acid, m hydroxybenzoic acid and p hydroxybenzoic acid in high performance capillary zone electrophoresis was investigated using cetyltrimethylammonium bromide(CTAB) as reversed reagent for electroosmotic flow The effects of pH of buffer solution and volume fraction of methanol on the separation, peak shape and elution order were studied