高效液相色谱法测定贝类中的软骨藻酸

High Performance Liquid Chromatographic Determination of Domoic Acid in Shellfish Samples

介绍了用反相高效液相色谱 (RP HPLC)测定贝类中软骨藻酸的方法。样品以V(甲醇 )∶V(水 ) =1∶1的溶液提取 ,经LC SAX强阴离子柱固相萃取净化 ,用RP HPLC定量分析。方法的最小检出限为 0 2 μg/ g ,在 1 0mg/L～ 2 5 0mg/L范围内有良好的线性关系 ,测定结果准确 ,重现性好 ,回收率大于 96 %。

Domoic acid in shellfish samples was determined by HPLC with DAD/UV detector at 242 nm Samples were extracted with methanol water followed by clean up of the extracts with strong anion exchange solid phase extraction cartridge(3 mL LC SAX) Zorbax S B C 18 column, 150 mm×4 6 mm i d , and mobile phase of acetonitrile 0.1% aqueous trifluoroacetic acid(13∶87, V/V )were used for the assay The quantitative analysis was performed with external standard The calibration curve of domoic acid was linear in the range of 1 0 m/L 25 0 mg/L and the detection limit was ca 0 2 μg/g