胃癌组织与正常组织样品FTIR研究

FTIR Study on the Normal and Cancerous Stomach Tissues

用傅里叶变换红外光谱仪研究胃癌组织及相应正常组织的红外光谱 ,结果表明癌组织样品与正常组织样品的红外光谱在峰形、峰强度、峰频率等方面均存在明显差异。讨论了磷酸二酯基团 (PO-2 )的对称和反对称伸缩振动 ;CH3、CH2 基团的伸缩及弯曲振动 ;C—O基团伸缩振动峰的变化。并从蛋白质、核酸氢键的角度以及膜脂碳氢链排列及构象变化角度分析了发生变化的原因。磷酸二酯基团 (PO-2 )的对称伸缩振动 νs,PO-2 呈现有规律性变化 ,即癌化后由平均 10 80 92cm-1向高波数方向位移到平均 10 85 93cm-1处 ,反对称伸缩振动νas,PO-2 由平均位于 12 39 6 4cm-1位移到平均位于 12 38 73cm-1处 ,表明核酸骨架上磷酸二酯基团中的氧原子形成氢键的程度加强了。另外膜脂分子中的CH2 弯曲振动δCH2 也呈有规律地向高波数方向移动 ,癌化后由平均位于 145 5 2 3cm-1位移到 145 7 37cm-1处 ,表明了膜脂双分子层结构中磷脂分子CH2 链无序程度加大了。细胞蛋白的C—O伸缩振动谱带νC—O 由平均位于 116 6 0 8cm-1位移到平均位于 116 6 5 8cm-1。

Tissues of cancerous and corresponding normal stomach were studied by FTIR technique. The results showed that there are obvious differences between FTIR spectra of them in spectral parameters such as frequency, intensity and band shape etc. The changes involving the phosphate symmetric stretching v(s,PO2)(-) and asymmetric stretching v(as,PO2)(-) modes, the CH(3) and CH(2) groups stretching (v(s,CH2), v(as,CH3)) and bending (delta (CH2)) modes and the C-O stretching v(C-O) mode were discussed. In addition, the changes of structure of hydrogen-bonding of nucleic acid and cell proteins and the packing and the conformational structure of the membrane lipids were analysed further. The average wavenumber of band of v(s,PO2)(-) shifted from 1 080.92 cm(-1) to 1 085.93 cm(-1) and that of v(as,PO2)(-).,pc shifted from 1 239.64 cm(-1) to 1 238.73 cm(-1) which indicated that the degree of hydrogen-bonding formed by oxygen atom of the phosphodiester groups of nucleic acids was increased. The average wavenumber of band of delta (CH2) of membrane lipids shifted from 1 455.23 cm(-1) to 1 457.37 cm(-1) that suggested that the conformational structure of the methylene chains of membrane lipids is more disordered than in normal tissues. The shift of band of v(C-O) of cell proteins from 1 166.08 cm(-1) to 1 166.58 cm(-1) indicated that the hydrogen-bond of cell proteins become weaker.