径向电场调制毛细管电泳法用于神经递质分离

Capillary Electrophoresis of Neurotransmitters Under the Control of Radial Electric Field

利用双向电场控制毛细管电泳系统，考察了神经递质的分离。在 ｐＨ ２．５的 ０．０１ｍｏｌ／Ｌ磷酸盐缓冲体系中，通过加入２０％（Ｖ／Ｖ）正丙醇，改善了多巴胺和５－羟色胺的分离效果，但仍不太理想。通过施加径向电场，可进一步提高分高度。本研究不仅拓宽了径向电场调控的样品分离范围，而且为生物活性物质的痕量分析提供了参考依据。

Separations of neurotransmitters such as dopamine (DA), serotonin (5-HA), norepinephrine (NE) and epinephrine (E) were performed successfully using a homemade electric field modulated capillary electrophoretic system, which could offer both radial and axial electric fields with only one high voltage power supply. DA and 5-HT were eluted simulaneously and could not be resolved in 0.01 mol/L phosphate buffer at pH 2.5. Alcohol additives, such as methanol, ethanol or l-propanol were added to the buffer to change the solvation shell of the solutes, which changed their effective sizes and electrophoretic mobilities of the solutes accordingly. The optimum composition was a buffer of 20% ( V/V) 1-propanol, with resulted resolutions 0.74 (DA/5-HT), 0.56(5-HT/NE) and 0.77 (NE/E). If a positive radial voltage of 6.6 kV was applied, the resolutions were improved to 1.48, 0.71 and 1.32, respectively.