手性固定相法拆分咔唑羧酸衍生物对映异构体

Enantiomeric Resolution of Carbazole Derivates with a Chiral Stationary Phase

用直链淀粉－三（３，５－二甲基苯基氨基甲酸酯）手性固定相，在正相条件下首次拆分了６种咔唑羧酸衍生物对映异构体。考察了流动相中不同的酸性添加剂、不同的王丙醇浓度对样品保留和拆分的影响。

Six pairs of enantiomeric compounds of carbazole derivatives were separated by high performance liquid chromatography using an amylose-tris (3, 5 - dimethylphenylcarbamate) chiral column made in our laboratory. The mobile phases were mixtrues of different content of n-hexane, n-propanol and a little amount of acidic additives. It was found that the stronger the acid added, the better the separation. All the enantiomers were baseline separated with a mobile phase comtaining 1 % trifluoroacetic acid and 5% n-propanol. The effect of sample structure on the retention factor was discussed. When the carboxylic chain of the sample became longer, the retention factor became smaller. When the sample was halogenated, the retention factor increased along with the increasing of the molecular weight of the samples.