加味温胆汤对SAM-P/10老化痴呆鼠三个脑区递质氨基酸的影响

The Influence of Xinnao Yizhi Prescription on IAAs in Hippocampi,Cortex and Striate Body of Brain of SAM-P/10

目的 :探讨中药加味温胆汤改善脑功能的中枢机制。方法 :采用高效液相技术系统观察SAM P 10鼠脑内皮层、海马、纹状体等与学习记忆关系密切的脑内 8种氨基酸水平的变化。结果 :加味温胆汤 1号方对于海马、纹状体异常升高的r GABA水平表现出明显降低作用。加味温胆汤预防方对皮层降低的Tau水平有明显升高 (P <0 0 1) ,1号方亦有明显升高 (P <0 0 5 ) ,2号方和西药仅表现上升趋势。结论 :加味温胆汤 1号对SAM P 10皮层、海马、纹状体的Clu等EAAS亢进状态有显著的调节作用 ,对于抑制性氨基酸IAAS的调节作用表现在对异常升高的r GABA水平的降低和使降低的Tau水平上升。

Objection:to explore the centre mechanism that Xinnao Yizhi prescription improve the function of brain. Methods:to use high performance liquid chromatography to observe the change of 8 amino acids of SAM-P/10's brain,which has close relation with learning memory. Results:preventive prescription and prescription No.I drpoed the level of abnormal increased r-GABA obviously (P<001 or 0.05),so did western medicine (P<005),prescription No.II had no obvious effect. Preventive prescription incrased the level of Tau in cortex significantly (P<001),so did prescription No.I (P<005),prescription No.II and western medicine only had increasing tendency. Conclusion:Prescription No.I that reinforces kidney,resolves phlegm,and restores a clear head regulates abnormal increased EAAs obviously,which is better than that of prescription No.II that reinforces kidney,remove stasis.Preventive prescription has the best effect and is better than western medicine. Preventive prescription that drop the level of r-GABA and reduce the level of Tau has the best effect. It prompts that Xinnao Yizhi prescription can regulated the level os SAM-P/10's EAAs and IAAs obviously,This provide bases for the clinical use of Xinnao Yizhi prescription on the encephalic senility and senile dementia.