深低温保存对脐血长期培养起始细胞含量的影响

Effect of cryopreservation on LTC-IC of umbillical cord blood

目的 :探讨深低温保存对脐血长期培养起始细胞 (L ong term culture initial cell,L TC- IC)含量的影响。方法 :利用 6 %羟乙基淀粉法分离脐血单个核细胞 ,采用基质细胞直接接触培养系统和 CFU- GM检测方法检测新鲜脐血和深低温保存的脐血中长期培养起始细胞和造血祖细胞。结果 :脐血深低温保存复苏后 ,CFU- GM由97± 5个 / 1× 10 5 MNC减少到 84± 13个 / 1× 10 5 MNC,回收率为 (83± 12 ) % ;L TC- IC87± 2 0个 / 1× 10 5 MNC减少到 6 8± 33个 / 1× 10 5 MNC,回收率为 (76± 33) %。结论 :深低温保存后 ,脐血中 L TC- IC的含量减少 ,但脐血仍保存大部分造血功能。

Objective:To explore the effect of cryopreservation on LTC IC counts of human umbilical cord blood.Method:Placental cord blood mononuclear cells were separeted by 6% hetastarch and cryopreserved in liquid nitrogen with 10% DMSO. LTC IC count and CFU GM were detected by stromal contact culture system and CFU GM culture system.Result:After thawing,CFU GM decreased from 97±5/1×10 5 MNC to 84±13/1×10 5 MNC,the recovery was (83±12)%; the number of LTC IC clone also decreased from 87±20/1×10 5MNC to 68±33/1×10 5 MNC,the recovery was (76±33)%.Conclusion:Cryopreservation could bring into some LTC IC lost,but have no influence on their major hematopoietic potentiality.