人巨细胞病毒对脐血巨核系祖细胞集落生长的影响

The effect of human cytomegalovirus on the colony formation of megakaryocyte progenitors

目的 :探讨人巨细胞病毒 (HCMV)对巨核系祖细胞 (CFU - MK)的分化和增殖的影响。方法 :收集 2 0例脐血标本 ,采用体外甲基纤维素半固体培养技术 ,观察 3种不同浓度 HCMV AD16 9株对 CFU - MK集落形成的影响。结果 :3个感染组的 CFU - MK集落数均减少 ,与灭活病毒组比较 ,分别为 2 4.0 %、35 .8%和 48.6 % (P <0 .0 5 ) ,显示抑制程度与病毒感染滴度呈剂量 -浓度依赖关系。结论 :HCMV在体外能抑制 CFU - MK的分化和增殖 ,提示巨细胞病毒感染引起的血小板减少与 CFU - MK受该病毒抑制有关。

Objective:To investigate the effect of HCMV on the differentiation and proliferation of megakaryocyte progenitors.Method:20 samples of cord blood were obtained,By the methylcellulose semi solid colony culture in vitro,3 the impact of 3 concentrations of HCMV AD169 on the CFU MK colony formation were surveyed.Result:Compared with the mock group,the CFU MK colonies decreased 24.0 %, 35.8 % and 48.6 %,respectively,in all the 3 infected groups (P< 0.05 ).It suggested that the suppression and the titer of virus was dose dependent.Conclusion:HCMV can inhibit the differentiation and proliferation of megakaryocyte progenitors in vitro. It suggests that thrombocytopenia caused by HCMV infection is related to the inhibition of megakaryocyte progenitors by this virus.