摘要
目的：探求清脑益智方治疗血管性痴呆或多发梗塞性痴呆的作用机理。方法：应用体外细胞培养的方法，观察中药对谷氨酸损伤神经元的影响。结果：清脑益智方可明显提高谷氨酸损伤后神经元的存活率（Ｐ＜０，０５）３降低孵育液中ＬＤＨ含量（Ｐ＜０．０５）；提高细胞生存活力（ＭＴＴ）；对星型胶质细胞的增殖没有影响（３Ｈ—ＴｄＲ）。结论：清脑益智方可能通过刺激星影胶质细胞分泌神经营养因子而起到对神经元的保护作用。
Objective: To explore the therapeutic mechanism of Qingnao Yizhi Formula (QYF) for vascular dementia and multi - infarct dermentia. Methods: In- vitro rabbit neuron culture was utilized to investigate the effect of QYF on glutamic - acid - induced neuron injury. Results: QYF could increase the survival rate of iniured neuron(P<0.05), decrease LDH content in the culture medium (P<0. 05) and increase the neuron survival activity (P<0. 05) in the culture medium added with MTT. Meanwhile, it had no effect on the proliferation of astrogliocytes. Conclusion: QYF capnrotect from glutamic - acid - induced injury by stimulating the secretion of neurotrophic factors from the astrogliocytes.
出处
《中药新药与临床药理》
CAS
CSCD
2001年第4期252-255,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家科委九五攻关项目(编号96-906-09-03)
关键词
清脑益智方
神经元损伤
化学诱导
谷氨酸
星形细胞
存活率分析
Qingnao Yizhi Formula /pharmacology
@Neuron Injury/chemically induced
@Glutamic-Acid/adverse effects
Astrocvtes/drug effects
Cerebrospinal fluid/drug effect
Survival analysis
In vitro
