肾康注射液对肾小球内皮细胞增殖及自分泌FN效应的影响

Effect of Shenkang Injection on Proliferation and Fibronectin Secretion of Glomerular Endothelial Cells

目的：探讨肾康注射液阻抑肾小球硬化、防治慢性肾功能衰竭的部分作用机理，为其临床应用提供依据。方法：采用血清药理学方法，观察肾康注射液对体外培养人肾小球内皮细胞增殖及其自分泌ＦＮ水平的影响。结果：肾康注射液及其含药血清对肾小球内皮细胞增殖及其自分泌 ＦＮ有明显抑制作用（Ｐ＜０．０１），且呈量效依赖关系，肾康注射液与肝素合用，协同抑制肾小球内皮细胞增殖、自分泌 ＦＮ水平（ Ｐ＜ ０． ０５）。结论：肾康注射液对肾小球内皮细胞的抑制效应，显示其新的药理作用位点。对肾小球内皮细胞增殖的抑制效应是肾康注射液活血功效的具体形式之一，对肾小球内皮细胞自分泌ＴＮ的抑制效应是其泄浊功效的具体形式之一。

Objective: To approach the mechanism of Shenkang Injection (SI) in prohibiting glomerulosclerosis (GS) and treating chronic renal failure (CRF). Methods: The proliferation and fibronectin (FN) level of cultured human glomerular endothelial cells (GEC) were detected by sero-phar macological test. Results: SI and SI serurm inhibit swiftcantly the proliferation and FN secretion of GEC in a dose- dependent manner (P<0.01); effect of SI was strengthened (P<0.05 ) when it is combined with heparin. Conclusion: GEC is the the pharmacological action site of SI. Inhibiting the proliferation of GEC and the production of FN may be one of the mechanisms of SI for CRF.