P-gp和GST-π在胆囊癌中的表达与化疗耐药的研究

Expression and chemotherapeutical resistance of P-glycoprotein and glutathione S-transferase in gallbladder carcinoma

目的 通过检测胆囊癌组织中P -糖蛋白 (P - gp)和胎盘型谷胱苷肽S转移酶(GST -π)的表达状况 ,揭示胆囊癌对化疗不敏感的内在机制。方法 38例胆囊癌组织石蜡标本 ,12例结石性胆囊炎组织石蜡标本分别进行生物素蛋白链菌素 -生物素 -过氧化物酶免疫组织化学染色 ,按照既定的阳性标准判定染色结果。结果 P - gp和GST -π在胆囊癌的表达阳性率分别为 78.9%和 6 5 .7% ,而在胆囊良性疾病中两者阳性率分别为 2 5 %和 16 .6 % ,两者比较均具有显著性差异 (P <0 .0 5 )。结论 P - gp、GST

Objective By approach the situations of expression of P-glucoprotein(P-gp) and placental glutathione S-transferase(GST- π) as a biomarker in gallbladder carcinoma(GC) to reveal the intrinsic mechanism of poor response of GC to chemotherapy. Method By means of the streptavidin-biotin-peroxidase complex staining method and according to the stand of positive-stainning,the expression of the P-gp and GST-π in tissues of GC and chronic calulous cholecystitis(CCC) were determined in 38 cases of GC and 10 cases of CCC. Result The positive rate of P-gp and GST-π were 78.9% and 65.7% respectively in GC and were higher that in benign(25% and 16.6%), the difference were significantly(P<0.05). Conclusions The higher expression of P-gp and GST- π may be the main reason of resistance of GC patients to chemotherapeutical agents.