同种异体反应性淋巴细胞克隆疫苗回输诱导受体特异性免疫耐受研究

Studies on the prolongation of cardiac allograft survival by alloreactive lymphocytes depletion. indaection of allograft immunotolerance by alloreactive lymphocyte vaccine in recipient preparation

目的 探讨同种异体移植抗原特异性免疫耐受诱导的新途径。方法 以同种异体反应性淋巴细胞克隆在体外培养条件下大量增殖后作为自身独特性疫苗 ,体内注射免疫家兔 ,以混合淋巴细胞反应来观察免疫前和免疫后对供体淋巴细胞刺激指数 (SI)的变化。结果 同种异体反应性自身独特型淋巴细胞疫苗具有显著诱导特异性免疫应答抑制现象 ,SI值显著降低 (P <0 .0 0 5 ) ,而对卡介苗诱导的特异性免疫应答并未因独特型淋巴细胞疫苗的应用下降。结论 同种异体反应性自身独特型淋巴细胞疫苗有可能成为诱导抗原特异性免疫耐受的一种手段 。

Objective Alloreactive lymphocytes specific for graft antigens are implicated in rejection of allograft.This study aims to induce alloreactive lymphocytes depletion or inactivation and allograft immuno tolerance by alloreactive lymphocyte vaccine(ALV).Methods Recipient lymphocytes were expanded for 9-14 days stimulated by donor lymphocytes that had been dealt with mitomycin C.The expanded recipient lymphocytes are termed alloreactive lymphocyte vaccine(ALV).About 0.5×10 7～1.0×10 7cells mixed with freunds complete ajuvant(FCA)were used as ALV to vaccinate itself for 3～4 times as usual. The stimulate index(SI) was measured after vaccination and compared with itself unvaccination. Results The ALV could suppress mixed lymphocyte reaction. The stimulate Index was significantly lower than themselves uninjected with ALV(P<0.005),while the stimulate Index of BCG(vacille camette guerin) was significantly higher than itself unvaccination with BCG.Conclusions ALV induced an effective anti-alloreactive lymphocyte response leading to a specific depletion or inactivation of alloreactive lymphocytes.The alloreactive specific immuno-tolearnce can be induced by immunization with attenuated auto-alloreactive lymphocyte vaccine.