LPS和TNF-α诱导血管内皮细胞ICAM-1蛋白表达的比较

A Comparison of LPS and TNF-α Induced ICAM-1 Expression of Vascular Endothelial Cells

目的和方法采用细胞间接免疫荧光染色和激光共聚焦显微镜扫描技术 ,研究脂多糖(LPS)和肿瘤坏死因子α(TNF -α)对人脐静脉内皮细胞 (HUVEC)表面细胞间粘附分子 -1(ICAM -1)表达的诱导作用。结果与内皮细胞表面ICAM -1的基础表达相比 ,LPS可诱导内皮细胞表面ICAM -1表达在第8～24h显著增加 ,但第36h有较明显的下降 ;TNF -α也可诱导内皮细胞表面ICAM -1表达在第8～24h显著增加 ,并在36h仍维持在较高水平。LPS和TNF -α与内皮细胞ICAM -1表达之间存在剂量反应关系。结论LPS和TNF -α可诱导血管内皮细胞ICAM -1的表达 ,但内皮细胞在表达ICAM -1时对LPS的长期刺激可能产生耐受性。

Objectve and methods To compare the difference of expression of intercellular adhesion molecule_1(ICAM_1) on human umbilical vein endothelial cell(HUVEC) induced by lipopolysaccharide(LPS) and tumor necrosis factor_α(TNF-α) by cellular immune fluorescent staining and confocal microscope. Results Comparing with basic expression of ICAM-1 on HUVEC, the ICAM-1 expression was enhanced significantly after 8 h to 24h of LPS stimulation, then descended at 36h obviously. the ICAM-1 expression induced by TNF-α was also increased significantly after 8h to 24h, and still maintained high level at 36h. Dose_dependent relationship existed between ICAM-1 expression and LPS or TNF-α. Conclusion The expression of ICAM-1 on HUVEC could be upregulated by LPS and TNF-α significantly, but tolerated to LPS chronical stimulation.