结直肠癌c-erbB-2和nm23癌基因蛋白的免疫组织化学研究

Immunohistochemical study of c-erbB-2 and nm23 proteins in human colorectal carcinoma

目的 根据结直肠癌的生物学行为 ,探讨结直肠癌手术标本中c erbB 2和nm2 3蛋白表达的临床意义及其术前检测c erbB 2和nm2 3蛋白对结直肠癌的诊断价值。方法 收集结直肠癌手术标本96例 ,其中 5 6例经肠镜活检标本病理证实 ,应用免疫组化技术检测c erbB 2和nm2 3蛋白在手术标本及活检标本中的表达。结果 c erbB 2阳性率为 2 1.4%～ 2 8.6 % ,nm2 3为 6 9.6 %～ 76 .1%。c erbB 2的表达与组织学类型有关 ,c erbB 2表达阳性组 5年生存率低于阴性组 ;nm2 3的表达与肿瘤的浸润深度Dukes′分期及组织学类型有关 ,且nm2 3表达阳性组 5年生存率高于阴性组。c erbB 2和nm2 3蛋白的阳性率在结直肠癌的肠镜活检标本和手术标本中无显著差异。结论 对结直肠癌组织进行c erbB 2和nm2 3蛋白的检测 ,有助于结直肠癌的诊断及非手术临床分期的判断 。

Objective The expression of c erbB 2 and nm23 proteins and its clinical significance was examined immunohistochemically in resected human colorectal carcinoma,to evaluate the roles of c erbB 2 and nm23 proteins in diagnosis of colorectal carcinoma.Methods The expression of c erbB 2 and nm23 proteins was detected immunohistochemically in 96 surgically resected specimens of colorectal carcinoma.Results The positive rate of c erbB 2 and nm23 immunoreactivity was 21.4%～28.6% and 69.6%～76.1% respectively,the presence of c erbB 2 was correlated with the histological types of patients with c erbB 2 positive tumor who had lower 5 year survival rate than that of negative.The expression of nm23 was negatively correlated with the depth of invasion Dukes′ stages and the histological types.Patients with nm23 positive tumor had higher 5 year survival rate than that of negative.The positive rates of c erbB 2 and nm23 had no statistical difference between biopsy and resected specimens.Conclusion Determination of c erbB 2 and nm23 proteins immunohistochemically might be useful in the diagnosis and clinical staging and predicting prognosis of colorectal carcinoma.