摘要
目的探讨CEA重组痘苗病毒(rV-CEA)治疗CEA+肿瘤的机制。方法以我室构建的rV-CEA, 腹腔接种供体C57/BL小鼠,取其脾细胞及腹腔巨噬细胞(MΦ),分别过继转移给荷CEA+-HePa肝癌细胞的C57/BL小鼠,检测该供体小鼠脾细胞、腹腔MΦ及相应受体的脾细胞体外杀瘤细胞的效应。结果接种rV-CEA的供体小鼠的脾细胞及腹腔MΦ过继免疫给受体小鼠,具有明显抑制受体CEA阳性肿瘤生长的作用。体外实验表明,该供体脾细胞及接种了供体MΦ的受体脾细胞对同一靶细胞的杀伤活性明显增强,但供体MΦ体外的细胞毒活性无明显增加。结论rV-CEA对CEA+肿瘤的抑制作用,可能主要通过CEA特异性免疫反应激活T细胞而实现。MΦ作为抗原提呈细胞可通过激活T细胞而杀伤肿瘤细胞,具体机制值得进一步研究。
:Aim To explore antitumor mechanism of a recombinant vaccinia virus containing the human CEA-cDNA (rV-CEA). Methods C57/BL mice were immunized three times with rV-CEA. Six weeks later, the macrophages(MΦs)and splenocytes from rV-CEA-immunized donors were transferred to CEA+-HePa tnmor-bearing recipients,Meanwhile, the antitumor effects of these donor's MΦs and splenocytes and that of the recipient's splenocytes were detected in vitro. Results The MΦs and splenocytes from rV-CEA-immunized donors possessed strong antitumor activity in CEA-positive tumor-bearing recipients. The in vitro antitumor effect of splenocytes from mice inoculated with MΦs from rV-CEA-immunized donors were markedly stronger than those from W-VV-immunized donors. However,the in vitro antitumor effect of the MΦs from rV-CEA-immunized donors was the same as those from W-VV-immunized donors. Conclusion It is demonstrated that antitumor activity induced with rV-CEA may be mediated mainly by antigen present cells (the MΦs), which activated tumor-specific T cells to kill tumor cells.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第4期324-327,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
广州市科委基金资助
No. JB02-98-5-018-01
广东省卫生厅资助课题
No.A1998127
广州医学院资助课题
No.2000-GK-14
关键词
癌胚抗原
重组痘苗病毒
免疫细胞
CEA阳性肿瘤
肿瘤抑制作用
carcinoembryonic antigen
recombinant vaccinia virus
immune cells
CEA positive tumor
antitumor effect