宫内注射胎肝细胞对大鼠移植免疫反应的影响

The effect of intrauterine injection of rat fetal hepatocytes on rat homograft rejection reaction

目的研究宫内注射胎肝细胞对大鼠移植免疫反应状态的影响。方法将LOU/CN大鼠的胎肝细胞注射于宫内CHN胎鼠的腹腔。以分娩7～9 wk龄时的CHN雌性大鼠作为受体，LOU/CN雌性大鼠作为供体，进行皮片移植。观察大鼠皮片移植物的存活时间，并以混合淋巴细胞培养检查移植排斥反应。结果与对照组相比较，宫内注射组大鼠皮片移植物的存活时间明显延长。混合淋巴细胞培养显示，移植排斥反应被明显抑制(P∨0.01)。结论胎肝细胞宫内注射于胎鼠可延长大鼠皮片移植物的存活期，对移植排斥反应产生明显的抑制作用。

Aim To investigate the effect of intrauterine injection of rat fetal hepatocytes on homograft rejection reaction. Methods The skin graft from a male LOU/CN rat was transplanted to a female recipient CHN rat of 7 to 9 weeks after parturition, and then survival time of the graft was observed. Simultaneously, homograft rejection reaction was examined by mixture lymphocyte culture. Results As compared with control group, survival time of transplanted skin graft was obviously prolonged. Mixure lymphocyte culture demonstrated that homograft rejection reaction was inhibited markedly. Conclusion Intrauterine injection of rat fetal hepatocytes wuld obviously inhibite homograft rejection reaction, thus prolonging survival time of the graft.