慈菇对镉致小鼠急性肝损伤的保护作用

The Protective Effect of Sagittaria Sagittifolia Against Cadmium-mediated Acute Liver Damage

目的 探讨慈菇对镉 (Cd)致小鼠急性肝损伤保护作用的机制。 方法 将 48只雄性昆明种小鼠 ,随机分为 6组 (n=8) ,连续染毒 5天后 ,比较各组血清脂质过氧化物 (L PO)、超氧化物歧化酶 (SOD)、铜蓝蛋白 (CP)浓度、肝匀浆谷胱甘肽 (GSH)及肝组织 Cd含量等指标 ,并检查肝脏病理学变化。 结果 3组慈菇干预组 L PO浓度 (nmol/ m l) 12 .2 2± 2 .0 9,13.47± 2 .6 8,12 .13± 3.0 1较阳性对照组 (18.0 3± 3.5 6 )显著降低 (P<0 .0 5 ) ,肝匀浆GSH含量 (m g/ g) 4 .83± 0 .44 ,5 .0 8± 0 .41,5 .2 2± 0 .39较阳性对照组 (4.0 1± 0 .6 7)显著升高 (P<0 .0 5 ) ,肝组织的病理损伤有所减轻 ,且肝组织 Cd含量与血清 L PO呈正相关 (r=0 .6 5 6 3,P<0 .0 1)。 结论 慈菇含有多种抗氧化成分 ,对 Cd导致的小鼠急性肝损伤有一定保护作用。

Objective\ To study the hepatoprotective effect of Sagittaria Sagittifolia against cadmium\|mediated acute liver damage.\ Methods\ 48 Kunming male one\|month\|old rats(divided into 6 groups randomly) were used for this experiment.\ After cadmium was given for five consecutive days,the concentration of serum LPO, SOD, CP and the content of liver Cd, GSH were measured in each group above, and the liver sections were examined pathologically also.\ Results\ The concentration of serum LPO in the groups treated with Sagittaria Sagittifolia(12 22±2 09, 13 47± 2 68, 12 13±3 01) was lower than that of the positive control(18 03±3 56), the liver GSH content(4 83±0 44, 5 08±0 41, 5 22±0 39) was higher than that of positive control(4 01±0 67), and the liver pathologic damage induced by cadmium was also alleviated.\ There was positive correlation between the liver cadmium content and the serum LPO concentration(r=0.6563,P<0.01).\ Conclusion\ Sagittaria Sagittifolia possesses protective effect against the cadmium\|mediated acute liver damage.\;