肝移植受者IL-10基因启动子多态性与急性排斥反应的关系

Relationship between IL-10 gene polymorphisms and acute rejection following liver transplantation

目的 探讨肝移植受者白细胞介素 10 (IL 10 )基因多态性与急性排斥反应的关系。方法 应用PCR限制性片段长度多态性分析法 ,检测 12 2例肝移植受者IL 10基因启动子的 2个多态位点 - 10 82和 - 5 92的各种基因型的分布 ,并分析它们与急性排斥反应的关系。结果 IL 10 - 5 92位点 ,A/A ,C/A ,C/C等基因型间的急性排斥反应发生率分别为 16.7%,19%,2 9%,相互比较 ,差异不显著 (P >0 .0 5 ) ;IL 10 - 10 82位点 ,尽管G/G基因型的急性排斥反应发生率 ( 3 3 %)高于A/A型( 18%) ,但差异仍不显著 (P >0 .0 5 )。结论 IL 10基因多态性与肝移植受者术后急性排斥反应无确切关系。

Objective To study the relationship between IL-10 gene polymorphisms and acute rejection following liver transplantation.Methods PCR restriction fragment polymorphisms assay was used to detect the distribution of genotypes, interleukin 10 (IL-10)-592, -1082 in 122 adult liver transplant recipients and its relation with the occurrence of acute rejection. All patients received standard immunosuppression with corticosteroids, azathioprine and cyclosporine or FK506.Results 31 (26%) patients developed acute rejection 1-18 weeks following transplantation. The acute rejection rate in liver transplant patients with A/A, C/A, C/C genotypes at IL-10 gene promoter -592 position was 1 6.7%,19% and 29%,respectively (P> 0.05); the acute rejection rate in liver transplant patients with G/G, G/A, A/A genotypes at IL-10 gene promoter -1082 position was 33%, 25% and 17.9%, respectively (P> 0.05).Conclusion There were no association between IL-10 gene polymorphisms and acute rejection following liver transplantation.