血瘀证动物细胞损伤模型的研制

A Preliminary Study on Establishment of Cellular Injury Models of Blood Stasis Syndrome

【目的】探索建立血瘀证细胞损伤模型的方法。【方法】用两种方法 ,即用血瘀证兔模型血管内皮细胞直接培养的方法和用血瘀证兔模型血清损伤培养的血管内皮细胞方法 ,研制血瘀证细胞损伤模型。【结果】第一种方法原代培养的血管内皮细胞出现了病理性损伤及内分泌功能的改变 ,初步表明能直接反映体内的病理状态 ,但其病理特征并未随细胞传代得以保留 ;第二种方法培养的细胞同样体现了类似前者的病理状态 ,且更易于复制。【结论】初步表明所建立的细胞损伤模型从功能和结构上都反映了血瘀证整体动物模型及部分临床病人的病理特征 ,可应用于探讨血瘀证实质和活血化瘀作用机理的研究。

Objective To explore methods for establishinga cellu lar injury model of blood stasis syndrome (BSS). Methods Two experiments were c arried out. In the first experiment,the injured vascular endothelial cell model of BSS was established by the culture of vascular endothelial cell, and in the seco nd experiment, the vascular endothelial cell injured by the serum of BSS rabbit was cultured. Results In the first experiment, pathological features and end ocrine dysfunction in the primary cultured endothelial cell were similar to the B SS rabbit model, but the above changes did not remain in the subculture. The above changes were also found in the second experiment and were easy to be repea ted. Conclusion The injured vascular endothelial cell model of BSS establis hed b y the above methods reflects or partially reflects the structure and function of BSS rabbit model and the patients with BSS, and can be used to study the pathol ogical mechanism of BSS and therapeutic effect of blood_circulation activating a nd blood_stasis removing herbs.