中国产海兔抗肿瘤活性成分研究(Ⅰ)

Studies on antineoplastic constituents from chinese marine mollusc sea hare(Ⅰ)

利用稻瘟霉生物活性筛选模型 ,经溶媒提取和多种层析方法 ,自蓝斑背肛海兔 (N otarchus leachiicirrosus)中追踪分离得到 2个抗癌活性成分。利用电喷雾离子质谱和核磁共振二维谱技术 ,分别将其鉴定为 :(7E) - 1-乙酸基 - 8-氯 - 7-二氯甲基 - 7-烯 - 4 -酮 - 3-甲基辛烷 ( ) ,(7Z) - 1-乙酸基 - 8-氯 - 7-二氯甲基 - 7-烯 - 4 -酮 - 3-甲基辛烷 ( )。化合物 I和 II均为首次从蓝斑背肛海兔中得到 。

Two active compounds were isolated from chinese marine mollusc sea hare (Notarchus leachii cirrosus) by bioassay guided method with conidia of Pyricularia oryzae , and by a combination of extraction and purification with suitable solvent as well as multiple column chromatographies. Their structures were elucidated as (7 E) 1 acetoxy 8 chloro 7 (dichloromethyl) 3 methyoct 7 en 4 one (Ⅰ), (7 Z) 1 acetoxy 8 chloro 7 (dichloromethy l) 3 methyoct 7 en 4 one (Ⅱ). All compounds were obtained for the first time from this specimen and showed significant antineoplastic activities in vitro .